Disparaged as “selfish DNA” for decades, it’s now clear that transposable elements (TEs) underlie the remarkably dynamic nature of bacterial, plant, and animal genomes. This conference builds on the highly successful 2021 and 2023 Mobile Genome EMBO workshops and has emerged as the major European venue in this ever-growing cross-disciplinary research field. The 2025 Mobile Genome conference will assemble recognized experts from diverse disciplines (e.g., genomics, epigenetics, structural biology, evolutionary biology, and developmental biology), as well as the next generation of scientists who will become leaders in the field, to discuss the broad impact of TEs on organismal biology. A dedicated discussion session focused on technological and conceptual advances will augment the program to provide a forum for cross-disciplinary interaction among attendees with the goal of catalyzing collaborative opportunities to address new questions and challenges in TE research.