Meetings/Workshops on Molecular Biology in Germany

This course will teach the biological researchers how to analyse biological data sets using open-source software. Most of the analysis will be performed with docker4seq package, which was developed to facilitate the use of computing demanding applications in the field of NGS data analysis. Docker4seq package uses docker containers that embed demanding computing tasks (e.g. short reads mapping) into isolated containers. This approach provides multiple advantages: the user does not need to install all the software on its local server, the results generated by different containers can be organised in pipelines and reproducible research is guarantee by the possibility of sharing the docker images used for the analysis.

EMBL Events;     Phone: [+49 6221 378 8797];     Email: events@embl.de

Tools for RNA-seq data analysis, Experimental design, Quality control, Normalisation and data reformatting, Basic statistics, Selecting differentially regulated genes/microRNAs, Selecting alternative splicing events, Multiple testing, Biological interpretation

Before the genetic information stored in DNA can be used to direct cell growth and metabolism, it has to be transferred into RNA. Messenger RNAs (mRNAs) that code for proteins and noncoding RNAs are key components in the transmission of genetic information in all life forms - from viruses to complex mammalian organisms. Exciting recent findings now reveal a new layer of information added to RNAs in the form of chemical marks (the epitranscriptome) that play a critical role in gene expression control. This remarkable complexity in the lives of RNAs will be discussed at this meeting.

EMBL Events;     Phone: [+49 6221 378 8797];     Email: events@embl.de

Transcription, RNA processing, Export and localisation, mRNA surveillance and decay, Translation and the control of mRNA expression by microRNAs, RNA modifications, Noncoding RNAs

Neuroepigenetics is a young field that is attracting a lot of attention. Genome-wide association studies and mouse models continue to discover new connections between chromatin regulators and neurodevelopmental disorders and epigenetic pathways have been implicated in various physiological and pathological aspects of brain function, such as memory formation and recovery, addiction and neurodegeneration. This conference aims to provide a gathering opportunity for researchers of all levels who are interested in how gene regulation participates in the development and function of the brain, with particular attention to the chromatin-based regulation of gene expression. The key objective is to bring together, in the same meeting, two groups of scientists: neuroscientists with interest in gene regulation and molecular biologists with strong expertise in the mechanisms of transcription and epigenetics that want to apply their knowledge to understanding how the brain works. We believe the two groups can learn a lot from each other and we hope this conference can function as a catalyst for scientific exchange and collaboration between them.

EMBL Events;     Phone: [+49 6221 378 8797];     Email: events@embl.de

Chromatin regulation during neurodevelopment, Epigenetics of cell identity in the brain, Transcriptional regulation of neuronal activity and plasticity, Gene regulation and behaviour, Epigenetic engineering and epigenomic mapping, Chromatin in brain disease

Biology,    Neurology

The analysis of single cells advances research in many fields. To properly deal with such minute amounts of sample, each step of the workflow is critical, from sampling to data analysis. Additionally, the integration of data from different approaches is becoming increasingly important to capture the whole picture. This course will cover established techniques from sampling through sequencing, protein analysis and data analysis. Samples will be collected via flow cytometry, gene expression will be analysed by qRTPCR and next generation sequencing, and protein analysis will be performed by proximity extension assays. In addition, alternative methods for the whole workflow will be covered in interactive sessions of lectures and Q&A with key experts in the field. Data collected by the different methods will be analysed during in-depth sessions, which are a strong component of the course.

EMBL Events;     Phone: [+49 6221 378 8797];     Email: events@embl.de

Single cell isolation with FACS, Single-cell genomics using Strand-Seq, Single-cell epigenetics using bisulfite sequencing, Microdroplet-based single-cell transcriptomics, Single cell NGS data analysis

Analysis,    Genomics and Bioinformatics

A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities and limitations of different purification and characterisation techniques and will address strategies to understand their role in biomedical applications.

EMBL Events;     Phone: [+49 6221 378 8797];     Email: events@embl.de

Extracellular vesicles, Extracellular vesicles characterisation, Extracellular vesicles composition, Extracellular vesicles and function