Conférences - Microscopie - Allemagne

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EMBL Course: Fundamentals of Widefield and Confocal Microscopy and Imaging
11 mai 2020 - 15 mai 2020 • Heidelberg, Allemagne
Résumé:
Light microscopy is the most widely used form of microscopy for life science research. The correct handling of the microscope and optimising image quality (e.g. contrast, resolution) as well as experimental design according to different applications are crucial to obtain the best results. This course will provide the participants with an overview about the basics of microscopy (light and optics, contrast techniques and imaging). The confocal part will include multicolor, 3D and time lapse imaging as well as spectral imaging, photobleaching (FRAP, FLIP, FLAP) and photoactivation techniques. Basic image analysis tools including measurement functions, colocalisation and spectral unmixing as well as 3D/4D image processing will be covered, both in theory and practice. The course will combine lectures with intense daily hands-on practices followed by discussions.
Contact:
EMBL Events;     Tél.: [+49 6221 378 8797];     Email.: events@embl.de
Sujets:
Basics of optics and microscopy, Contrast methods, Basics of digital imaging, Fluorescence microscopy, Hands-on sessions
Identifiant de l'évènement:
1274357
Sujets apparentés:
2
EMBL Course: Advanced Fluorescence Imaging Techniques
24 mai 2020 - 29 mai 2020 • Heidelberg, Allemagne
Résumé:
This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with experts from the EMBL and microscopy professionals will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain quantitative information about protein dynamics in living samples.
Contact:
EMBL Events;     Tél.: [+49 6221 378 8797];     Email.: events@embl.de
Sujets:
Time-lapse microscopy, Confocal microscopy, Total internal reflection fluorescence microscopy (TIRFM), Super resolution microscopy (PALM, STORM), Expansion microscopy, Fluorescence resonance energy transfer (FRET), Fluorescence recovery after photobleaching (FRAP), Photoconversion, Photoactivation
Identifiant de l'évènement:
1274312
3
EMBO Practical Course: Advanced Electron Microscopy for Cell Biology
16 jui 2020 - 26 jui 2020 • Heidelberg, Allemagne
Résumé:
This course offers the opportunity to acquire a portfolio of cutting edge EM methods; allowing the participants to integrate EM tools and readouts into the workflow of their own cell biological research projects. The course focuses on teaching the theoretical background and the latest practical developments in the field.
Contact:
EMBL Events;     Tél.: [+49 6221 378 8797];     Email.: events@embl.de
Sujets:
State of the art fixation including cryo-methods, Correlative light and electron microscopy, Molecular mapping (immuno-EM), Three-dimensional EM imaging using tomography and serial imaging, Advanced quantitative image analysis
Identifiant de l'évènement:
1274385
Sujets apparentés:
4
EMBL Course: Cryo-Electron Microscopy and 3D Image Processing
23 aou 2020 - 31 aou 2020 • Heidelberg, Allemagne
Résumé:
Cryo-EM combined with image processing is currently undergoing what has been referred to as a “resolution revolution”, making it one of the most powerful techniques to address this challenge. It also has unique capabilities to obtain structural information in situ within complex environments, and to deal with heterogeneous samples. The methods are complex and under constant development, so in depth, advanced training of users is essential to ensure future users can adapt new technologies to expand into new areas and solve new biological problems. This course provides the theoretical and practical training covering the full breadth of cryo-electron microscopy (cryo-EM) techniques in order develop novel image processing approaches.
Contact:
EMBL Events;     Tél.: [+49 6221 378 8797];     Email.: events@embl.de
Sujets:
Single particle reconstruction, Subtomogram averaging, Single particle helical reconstruction, Atomic model interpretation, EM databank
Identifiant de l'évènement:
1274414
5
EMBL Course: Gene Expression at Spatial Resolution
31 aou 2020 - 04 sep 2020 • Heidelberg, Allemagne
Résumé:
This course will introduce participants to a new generation of a spatially resolved transcriptomics assay provided by 10x Genomics. Through the combination of state-of-the-art sequencing technology and high-resolution microscopy, 10x Genomics is enabling researchers to study gene expression of cells within their morphological context of an intact tissue section. Through both theoretical sessions, as well as extensive hands-on practical work, the participants will learn the entire workflow of sample preparation including cryosectioning of fresh-frozen tissue samples, optimisation of tissue fixation and permeabilisation, high-resolution microscopy and sequencing library preparation. Utilising the 10x Genomics Loupe Browser pipeline, the participants will also have hands-on training of spatial transcriptomics data analysis.
Contact:
EMBL Events;     Tél.: [+49 6221 378 8797];     Email.: events@embl.de
Identifiant de l'évènement:
1274457
Sujets apparentés:


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Dernière mise à jour: 23 Septembre 2019