The fundamental question of the role of metabolism in development has seen a revival in interest over recent years, creating the need for the first edition of this workshop in 2023. This second edition is set to reunite the community two years on, and to ensure the ongoing interactions that can propel progress in developmental metabolism. In this rapidly advancing field, metabolomic methods play a crucial role in unraveling complex processes. The workshop will again aim to bring together researchers from diverse backgrounds: developmental biology, genetics, epigenetics, ecology, physics and math, to provide the developmental metabolism community with cutting-edge research and methodologies applied across scales, insights into the latest advancements in metabolomic techniques and their application to developmental biology. New for 2025 will be an increased integration of tool development and technologies and an added focus on novel metabolomic approaches.